This web page was produced as an assignment for Gen677 at UW-Madison Spring 2013
Microarray
Figure 1. Example of a microarray [2]
Microarrays are a molecular tool used to measure the expression levels of hundreds of genes in a cell by measuring mRNA levels [1]. Microarrays work by having a oligonucleotide probe attached to a solid surface and then washing a nucleic acid target from a sample across the surface to see if the probe and the target hybridize [1]. Florescent tags are attached to the target so that hybridization can be visualized [1]. This technique allows researchers to assay a very large number of genes and their expression levels very quickly as well as compare expression levels between different cell or tissues types under different treatment conditions [1].
Microarrays of MYO5a Expression
All MYO5a microarray data was viewed using the Gene Expression Omnibus (GEO) database, a database that complies microarray and sequence based data [3]. No relevant microarray data was found for MYO5a on the GEO database. MYO5a expression has been included in studies profiling expression levels for different kinds of cancer and other disease states such as Huntington's but no MYO5a directed microarray studies were found [3].
References
[1] DNA Microarray Technology. National Human Genome Research Institute. Retrieved May 15, 2013 from http://www.genome.gov/10000533
[2] UNC Microarray Database. Retrieved May 15, 2013 from https://genome.unc.edu/
[3] Gene Expression Omnibus. NCBI. Retreived May 15, 2013 from http://www.ncbi.nlm.nih.gov/geo/
[2] UNC Microarray Database. Retrieved May 15, 2013 from https://genome.unc.edu/
[3] Gene Expression Omnibus. NCBI. Retreived May 15, 2013 from http://www.ncbi.nlm.nih.gov/geo/