This web page was produced as an assignment for Gen677 at UW-Madison Spring 2013
Protein Interaction Webs
A protein-protein interaction is any situation where one protein binds to another to carry out a function in the cell [1]. These interactions can be identified using methods such as yeast two hybrid, TAP tag, coimmunoprecipitation and affinity chromatography to name a few [1]. Protein interaction webs are diagrams that show the different proteins-protein interactions of a particular protein of interest. In the web the protein of interest is at the center and is connected to all proteins that it directly interacts with which are located on the periphery. The different interactions between periphery proteins are also shown. Analysis of these webs can reveal unexpected protein interactions and patterns of interaction. This can be critical information for directing a topic of research by revealing unexpected patterns and connections. All of the following protein interaction webs for Myosin Va were created using String [2].
What proteins interact with Myo5a?
Three different protein interaction webs were analyzed using String, horse, human and zebrafish. In each of the three webs different distinct groups of proteins could be seen associateding the MYO5a. A group of apoptosis proteins was found in all three webs. Each web also had distinct types of groups identified including melanosome transport, signal transduction, DNA repair and organelle biogenesis. The horse interaction web includes few proteins as expect since the horse is not a model organism and has not been much studied.
|
Analysis and Discussion
After analyzing the different protein interaction webs for the zebrafish, horse and human what became very clear was that few of the proteins identified to interact with MYO5a were related to neuron function. A few synaptic trasmission proteins were found in the human interaction web but this was all. Since MYO5a is very important in neuronal protein and vesicle transport it is interesting that so few interacting neuron proteins have been identified. This presents an interesting area for further study into MYO5a neuron function. Once the proteins that associate with MYO5a in neurons are identified MYO5a function in those neurons can be better understood. With a better understanding of this critical function it may be possible to begin looking for treatment options for those suffering from MYO5a neurological diseases.
References
[1] Phizicky EM, and Fields S,. (1995) Protein-protein interactions: Methods for detection and analysis. Microbiology Review. 59, 94-123
[2] String - Known and Predicted Protein-Protein Interactions. Retrieved May 14, 2013, from http://string-db.org/
[2] String - Known and Predicted Protein-Protein Interactions. Retrieved May 14, 2013, from http://string-db.org/
Rebecca Blackburn, [email protected], 5/14/13